In vivo microbicide data
An implant designed to release tenofovir at 40 µg/day was inserted into femail Dutch Belted rabbits. The implants remained in place for 14 days. Cervico-vaginal lavage (CVL) was performed by instilling sterile normal saline into the vagina and aspirating the pooled fluid using a syringe and quill. Plasma samples were obtained before and periodically after implantation. Tenofovir levels were measures using HPLC. Following the final specimen, the animals were euthanized, and the genital tract was excised to obtain tissue levels of tenofovir.
Vaginal tenofovir levels in rabbits
In a collaboration with the CDC, monkey rings designed to release 160 ug/day of tenofovir were inserted into pigtail macaques. Tenofovir levels in the blood, tissue and vaginal fluid were examined as will as cytokine composition and vaginal microflora. No evidence of toxicity was observed.
Vaginal tenofovir levels in macaques